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FAQ

Posted on February 2, 2024
What kinds of positive controls are necessary for calcium assays?
Calcium IndicatorsIntracellular IonsPhysiological ProbesRatiometric Calcium Indicators


Posted on February 2, 2024
Are there any reasons for using flow cytometry to measure calcium flux?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
Can I use the same calcium reagents for microscopy as I do for flow?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsMicroscopyPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
Is it possible to measure real time calcium flux with a flow cytometer?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
Can I measure what proportion of cells are responsive in my calcium flux assay?
Calcium IndicatorsEC50Intracellular IonsPhysiological ProbesRatiometric Calcium Indicators


Posted on February 2, 2024
What are the advantages of using a ratiometric calcium probe vs. a single-channel dye?
Calcium IndicatorsIntracellular IonsPhysiological ProbesRatiometric Calcium Indicators


Posted on February 2, 2024
Which calcium dyes work with flow cytometry lasers?
Calcium IndicatorsFlow Cytometry ReagentsFluoresceinsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
Can I combine Fura Red with Fluo-3 or Fluo-4 for ratiometric analysis?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
Should cells be equilibrated before a flow calcium assay?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
What kinds of stimulants are used for flow calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
What is the purpose of ionomycin in calcium flux flow assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
What buffers should be used for flow calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
What is the difference between negative control and baseline for flow cytometry calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
Are there any substitutes for probenecid in calcium assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometryprobenecid


Posted on February 2, 2024
Is probenecid required for calcium assays?
Calcium IndicatorsIntracellular IonsPhysiological ProbesRatiometric Calcium Indicatorsprobenecid


Posted on February 2, 2024
What is the purpose of probenecid in calcium flux flow assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometryprobenecid


Posted on February 2, 2024
Is a wash step required for calcium flux flow assays?
Calcium IndicatorsFlow Cytometry ReagentsIntracellular IonsPhysiological ProbesRatiometric Calcium IndicatorsSpectral Flow Cytometry


Posted on February 2, 2024
What is the dye concentration for calcium indicators?
Calcium IndicatorsIntracellular IonsPhysiological ProbesRatiometric Calcium Indicators


Posted on February 2, 2024
Are there any red calcium flux dyes?
Calcium IndicatorsIntracellular IonsPhysiological ProbesRatiometric Calcium Indicators


Posted on July 29, 2021
What is ratiometric imaging?
Ratiometric Calcium IndicatorsCalcium IndicatorsPhysiological ProbesIntracellular Ions


Posted on July 29, 2021
Why is Fura-2 unable to cross cell membranes?
Ratiometric Calcium IndicatorsCalcium IndicatorsIntracellular IonsPhysiological Probes


Posted on July 29, 2021
What is 2-photon calcium imaging?
Ratiometric Calcium IndicatorsCalcium IndicatorsIntracellular Ions


Posted on July 29, 2021
How does Fura red work?
Ratiometric Calcium IndicatorsCalcium IndicatorsCell Signaling


Posted on July 26, 2021
What kind of microscope do I need for ratiometric dyes?
Ratiometric Calcium IndicatorsCalcium IndicatorsIntracellular IonsPhysiological Probes


Posted on July 23, 2021
What is the difference between Fura-2 and Quin-2
Calcium IndicatorsIntracellular IonsRatiometric Calcium Indicators


Posted on July 23, 2021
What is a dual excitation dye?
Calcium IndicatorsRatiometric Calcium IndicatorsPhysiological Probes


Posted on July 23, 2021
What is the difference between ratiometric imaging and calcium imaging?
Calcium IndicatorsRatiometric Calcium IndicatorsPhysiological Probes


Posted on July 23, 2021
What is a dual-wavelength ion indicator?
Ratiometric Calcium IndicatorsCalcium IndicatorsPhysiological Probes


Posted on July 23, 2021
What are ratiometric dyes?
Ratiometric Calcium IndicatorsCalcium IndicatorsPhysiological Probes


Posted on July 23, 2021
What is a ratiometric indicator?
Ratiometric Calcium IndicatorsCalcium IndicatorsPhysiological Probes


Posted on July 23, 2021
What is ratiometric fluorescence used to detect?
Ratiometric Calcium IndicatorsCalcium IndicatorsPhysiological Probes


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